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Animalia >Mollusca >Gastropoda >Neogastropoda >Muricidae >Rapana >

0. Helper: Yukihiko MATSUYAMA, Shoji NAKANO, Kazumasa HASHIMOTO, Tatsuya YURIMOTO, Sou NAGASOE, Seiei KANEMATSU, Atsushi ITO (Fisheries Research Agency)

1. Rapana venosa
a) Sampling area: Oura, the Ariake Sea, Japan
b) Sampling date: 20 April 2020
c) Body size: 81.9 mm in shell height, 58.4 mm in shell width
d) Acclimation: the gastropod had been kept in an aquarium with sea water flowing (about 21 ˚C) until 20 May 2020. Fed with the mussel Mytilus galloprovincialis.

2. Atrina sp.
a) Source: a specimen sampled from the artificial seeds that had been produced from the spawning of Atrina sp. on 30 June and 11 July 2017
b) Body size: 87.6 mm in shell length, 42.4 mm in shell height
c) Method for rearing: kept in a cage fixed on the tidal flat in the Ariake Sea

3. Apparatus for observation
a) Round aquarium: 30 L in volume, 38 cm in diameter, 30 cm in height
b) Sediment: anthracite (2 mm in grain size)
c) Volume of sediment and seawater: the aquarium was filled with anthracite from the bottom to the 10 cm height and further with running seawater to 21 cm height.

4. Experimental condition
a) Light: 11 ¦Ģ mol/m2/s
b) Water temperature”§ 23.0 ˚C
c) Salinity: 33
d) Aeration: none
e) Flow of seawater: made

5. Time lapse camera
a) ModelӤ Brinno TLC 200 Pro
b) SettingӤ focus = 20 cm, frame rate = 20 fps, white balance = 2,800 inc., scene = twilight, HDR = high, interval = 2 seconds

6. Schedule
a) 20 April 2020: catch of the gastropod at Oura, the Ariake Sea
b) 24 April: transport of the gastropod from Oura to Seikai National Fisheries Research Institute
c) 24 April to 20 May: acclimation of the gastropod
d) 13:50, 20 May: the pen shell was transplanted on the anthracite in the 30-L aquarium
e) 14:39, 20 May: the gastropod was moved into the 30-L aquarium
f) 14:39, 20 May to 10:10, 21 May: photographing (only the photographs recording the predation of the pen shell were uploaded)

7. Acknowledgements
Predatory animals were caught by Saga Ariake Fishery Cooperative Oura Branch and Shimabara Fishery Cooperative. Seed production of the pen shell indispensable for the present study was assisted by Nagasaki Prefectural Institute of Fisheries, Saga Prefectural Ariake Fisheries Research and Development Center, Fukuoka Fisheries and Marine Technology Research Center Ariakekai Laboratory, and Kumamoto prefectural Fisheries Research Center. The present study was financially supported by contract work with Ministry of the Environment, Japan. I am grateful to all of them.

(Data No.momo200728rv01b)

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